Gdnf CLIA Kit for Mouse(Mus musculus) | EIAab.comGdnf CLIA Kit for Mouse(Mus musculus) | EIAab.com

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Gdnf (Gene name),

Glial cell line-derived neurotrophic factor (Protein name ), GDNF_MOUSE from NCBI database.

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General Annotation

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Antigen Annotation

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3D

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Predicted Eptitope

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Vaild Sequence

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Related Databases

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Gene name:

Gdnf;

Protein name:

Glial cell line-derived neurotrophic factor(mGDNF);

Alternative:

Astrocyte-derived trophic factor(ATF);

Organism:

Mouse (Mus musculus).

General Annotation

Sub Unit:

Homodimer; disulfide-linked.

Function:

Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

Subcellular Location:

Secreted

Protein Attributes:

Sequence length:

211

Sequence:

50:

MKLWDVVAVC | LVLLHTASAF | PLPAGKRLLE | APAEDHSLGH | RRVPFALTSD |

100:

SNMPEDYPDQ | FDDVMDFIQA | TIKRLKRSPD | KQAAALPRRE | RNRQAAAASP |

150:

ENSRGKGRRG | QRGKNRGCVL | TAIHLNVTDL | GLGYETKEEL | IFRYCSGSCE |

200:

SAETMYDKIL | KNLSRSRRLT | SDKVGQACCR | PVAFDDDLSF | LDDNLVYHIL |

211:

RKHSAKRCGC | I

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

Pfam:

String:

UniGene:

KEGG:

SMR:

Uniprot:

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ELISA Kit for Mouse mGDNF

Cat.:

E0043r

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96T

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ELISA Kit for Mouse mGDNF

Cat.:

E0043h

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96T

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ELISA Kit for Mouse mGDNF

Cat.:

E0043m

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CLIA Kit for Mouse mGDNF

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U0043m

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CLIA Kit for Mouse mGDNF

Cat.:

U0043h

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96T

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CLIA Kit for Mouse mGDNF

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U0043r

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96T

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Polyclonal Antibody for Mouse mGDNF

Cat.:

P0043Rb-h

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse mGDNF

Cat.:

P0043Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Mouse mGDNF

Cat.:

P0043Rb-r

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Packing:

40ug/0.2ml

Monoclonal Antibody for Mouse mGDNF

Monoclonal Antibody for Mouse mGDNF

Monoclonal Antibody for Mouse mGDNF

Protein for Mouse mGDNF

Protein for Mouse mGDNF

Protein for Mouse mGDNF

R&D Technical Data

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s

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Precision

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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Intra-Assay CV: ≤4.3%

Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.

Inter-Assay CV: ≤7.5%

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Recovery

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Recovery was determined by spiking various levels of GDNF into serum and plasma .

Sample Type	Average(%)	Recovery Range(%)
Serum	105	102-108
Plasma	106	99-107

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Linearity

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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDNF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1：2	1:4	1:8	1:16
serum(n=5)	102-106%	101-107%	96-103%	93-98%
EDTA plasma(n=5)	97-105%	104-108%	99-103%	89-96%
heparin plasma(n=5)	104-106%	107-110%	95-99%	100-105%

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References

1.

"Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine growth-promoting activities."
Watabe K. , Fukuda T. , Tanaka J. , Honda H. , Toyohara K. , Sakai O.
J. Neurosci. Res.41:279-290(1995) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
strain: ICR.
tissue: Spinal ganglion.
2.

"Embryonic expression of glial cell-line derived neurotrophic factor (GDNF) suggests multiple developmental roles in neural differentiation and epithelial-mesenchymal interactions."
Hellmich H.L. , Kos L. , Cho E.S. , Mahon K.A. , Zimmer A.
Mech. Dev.54:95-105(1996) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];TISSUE SPECIFICITY;DEVELOPMENTAL STAGE
strain: 129/SvJ.
tissue: Glial cell.
3.

"Cloning and structural organization of the gene encoding the mouse glial cell line-derived neurotrophic factor, GDNF."
Matsushita N. , Fujita Y. , Tanaka M. , Nagatsu T. , Kiuchi K.
Gene203:149-157(1997) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3);NUCLEOTIDE SEQUENCE [GENOMIC DNA];INDUCTION
tissue: Neonatal brain.
4.

"Cloning and characterization of mouse glial cell line-derived neurotrophic growth factor (GDNF)."
Wang F.-J. , Zhao J. , Too H.P.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao30:375-381(1998) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3);PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA];NUCLEOTIDE SEQUENCE [MRNA] OF 1-103 (ISOFORM 2);TISSUE SPECIFICITY
strain: BALB/c.
strain: C57BL/10J.
tissue: Brain.
tissue: Glial cell.
5.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3)
tissue: Brain.

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